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KMID : 0644120120250030016
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Journal of Oriental Gynecology
2012 Volume.25 No. 3 p.16 ~ p.26
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Effects of Cordyceps militaris(CM) on Osteoclastogenesis and Gene Expression
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Choi Kyung-Hee
Yoo Jeong-Eun
Hwang Bang-Yeon
Yoo Dong-Youl
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Abstract
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Objectives: This study was performed to evaluate effects of Cordyceps militaris(CM) on osteoclast differentiation and its related gene expression.
Methods: We used mouse myeloid cells RAW 264.7 stimulated by receptor activator of nuclear factor kappa-B ligand(RANKL) to induce osteoclast differentiation. There are four groups of which RAW 264.7 cells are not stimulated by RANKL (Normal), stimulated by RANKL without CM(Control), stimulated by RANKL with 0.1 of CM(CM 0.1), stimulated by RANKL with 1 of CM(CM 1). Osteoclastogenesis was measured by counting Tartrate-resistant acid phosphatase-positive multinucleated cells [TRAP(+) MNC]. RT-PCR was performed to evaluate the inhibitory effect of CM on gene expression(TRAP, AKT1, JNK1, NFATc1, c-Fos, MITF).
Results: 1. CM decreased the number of TRAP(+) osteoclast in RANKL-stimulated RAW 264.7 cell at the concentration of 0.1 and 1 . 2. CM decreased the expression of TRAP in osteoclast at the concentration of 1 . 3. CM decreased the expression of AKT1, JNK1 in osteoclast at the concentration of 1 . 4. CM didn’t affect the expression of NFATc1, c-Fos, MITF in osteoclast.
Conclusions: Cordyceps militaris has inhibitory effects on osteoclast differentiation and its related gene expression. These results suggest that Cordyceps militaris has a potential as a treatment of osteoporosis.
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KEYWORD
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Cordyceps militaris, Osteoclast, Osteoporosis, Osteoclastogenesis
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